Application

Functional Genomics/Transgenic Applications Creation of gene knockouts Creation of knock-in animals or cell lines with promoters, fusion tags or reporters integrated into endogenous genes

Features and Benefits

HPLC-purified SygRNA^™ Cas9 tracrRNA oligo is optimized for use with a target-specific cRNA and a source of SpCas9 protein for genome editing applications.

General description

The SygRNA^™ synthetic RNA system recruits SpCas9 nuclease to specific genomic targets. The tracrRNA (trans-activating crRNA) binds to a crRNA (CRISPR RNA) and the resulting crRNA-tracrRNA complex guides Cas9 protein to crRNA-specified sites in the genome. SygRNA^™ synthetic tracrRNA and crRNAs are optimized for use with Cas9 expressing of cell lines, Cas9 protein, or Cas9 mRNA and compatible with a variety of delivery methods such as microinjection, lipofection, and electroporation.

Legal Information

CRISPR Use License Agreement

SygRNA is a trademark of Sigma-Aldrich Co. LLC

Linkage

The SygRNA^™ Cas9 tracrRNA oligo is used in conjunction with a target-specific crRNA and a source of Cas9 protein such as TGEN-CP-50UG, CAS9P, or CAS9MRNA to form active RNP complexes. Select predesigned unique crRNAs against human, mouse and rat genes at CRISPR search.Learn more about SygRNA^™ tracrRNANeed custom tracrRNA ?

Physical form

Dry

Preparation Note

Resuspend SygRNA^™ Cas9 tracrRNA oligo in a RNAase-free Tris-containing buffer with a pH 8.0 or less.